13 Publications

Ayuso-Fernández I, Ruiz-Dueñas FJ, Martínez AT

[2018] Evolutionary convergence in lignin-degrading enzymes
Proc. Natl. Acad. Sci. USA, 115: 6428-6433
We analyze the molecular mechanisms that led to the rise of a powerful strategy for lignin degradation (i.e., the formation of a solvent-exposed tryptophanyl radical capable of oxidizing the bulky lignin polymer) as a convergent trait in different species of fungi (order [...]

Martínez AT, Camarero S, Ruiz-Dueñas FJ, Martínez MJ

[2018] Biological Lignin Degradation
Lignin Valorization: Emerging Approaches. Ed. Gregg Beckham. RSC: 199-225
In nature, white-rot fungi and some bacteria secrete powerful oxidative enzymes such as peroxidases and laccases to break down lignin and lignin products. These enzymes, together with different chemical mediators, form a chemo-enzymatic system for delignifying biomass so [...]

Serrano L, Esakkimuthu SE, Marlin N, Brochier-Salon MC, Mortha G, Bertaud F

[2018] Fast, Easy, and Economical Quantification of Lignin Phenolic Hydroxyl Groups: Comparison with Classical Techniques
Energy&Fuels, 32: 5969-5977
The quantification of lignin functional groups constitutes an essential step for effective assessment of lignin transformation. A new, fast, economical, and simple method without the use of harmful solvents is presented for lignin phenolic hydroxyl group quantification. [...]

Hämaläinen V, Grönroos T, Suonpää A, Heikkilä MW, Romein B, Ihalainen P, Malandra S, Birikh KR

[2018] Enzymatic process to unlock the lignin value
Front. Bioeng. Biotechnol., 10.3389/fbioe.2018.00020
Main hurdles of lignin valorization are its diverse chemical composition, recalcitrance, and poor solubility due to high-molecular weight and branched structure. Controlled fragmentation of lignin could lead to its use in higher value products such as binders, coatings, [...]

Rodríguez-Escribano D, de Salas F, Pardo I, Camarero S

[2017] High-Throughput Screening Assay for Laccase Engineering toward Lignosulfonate Valorization
Int. J. Mol. Sci., 18: 1793-1803
Lignin valorization is a pending issue for the integrated conversion of lignocellulose in consumer goods. Lignosulfonates (LS) are the main technical lignins commercialized today. However, their molecular weight should be enlarged to meet application requirements as additives [...]

Martínez AT, Ruiz-Dueñas FJ, Camarero S, Serrano A, Linde D, Lund H, Vind J, Tovborg M, Herold-Majumdar OM, Hofrichter M, Liers C, Ullrich R, Scheibner K, Sannia G, Piscitelli A, Pezzella C, Sener ME, Kılıç S, van Berkel WJ, Guallar V, Lucas F, Zuhse R, Ludwig R, Hollmann F, Fernandez-Fueyo E, Record E, Faulds CB, Tortajada M, Winckelmann I, Rasmussen J, Gelo-Pujic M, Gutiérrez A, del Río JC, Rencoret J, Alcalde M

[2017] Oxidoreductases on their way to industrial biotransformations
Biotechnol. Adv., 35: 815-831
Fungi produce heme-containing peroxidases and peroxygenases, flavin-containing oxidases and dehydrogenases, and different copper-containing oxidoreductases involved in the biodegradation of lignin and other recalcitrant compounds. Heme peroxidases comprise the classical [...]

Lucas F, Monza E, Jørgensen LJ, Ernst HA, Piontek K, Bjerrum MJ, Martínez AT, Camarero S, Guallar V

[2017] Simulating Substrate Recognition and Oxidation in Laccases: From Description to Design
J. Chem. Theory Comput. , 13: 1462-1467
To meet the very specific requirements demanded by industry, proteins must be appropriately tailored. Engineering laccases, to improve the oxidation of small molecules, with applications in multiple fields, is, however, a difficult task. Most efforts have concentrated on [...]

Bertaud F, Lichère H, Serrano L, Mortha G, Marlin N

[2017] Determination of functional groups of industrial lignins as key indicators for their use as novel green ingredients
4th International Symposium on Green Chemistry
In order to promote the recovery and use of industrial lignins from black liquor in pulp and paper industry, the reactivity and physico-chemical properties of several industrial lignins were evaluated. The recent development of a new conductometric/pH metric method was demonstrated [...]

Ayuso-Fernández I, Martínez AT, Ruiz-Dueñas FJ

[2017] Experimental recreation of the evolution of lignin-degrading enzymes from the Jurassic to date
Biotechnol. Biofuels, 10: 67
Background Floudas et al. (Science 336: 1715) established that lignin-degrading fungi appeared at the end of Carboniferous period associated with the production of the first ligninolytic peroxidases. Here, the subsequent evolution of these enzymes in Polyporales, where [...]

de Salas F, Pardo I, Salavagione HJ, Aza P, Amourgi E, Vind J, Martínez AT, Camarero S

[2016] Advanced Synthesis of Conductive Polyaniline Using Laccase as Biocatalyst
PLoS ONE, 11: 164958
Polyaniline is a conductive polymer with distinctive optical and electrical properties. Its enzymatic synthesis is an environmentally friendly alternative to the use of harsh oxidants and extremely acidic conditions. 7D5L, a high-redox potential laccase developed in our [...]